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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
17/04/2024 |
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Actualizado : |
17/04/2024 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
AREVALO, A.P.; BASIKA, T.; ANCHETA, S.; PERELMUTER, K.; RICCIARDI, A.; BOLLATI-FOGOLÍN, M.; CRISPO, M. |
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Afiliación : |
ANA PAULA AREVALO, Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; TATIANA BASIKA, Unidad Mixta entre Instituto Pasteur de Montevideo e INIA (UMPI), Montevideo, Uruguay; Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; Institut Pasteur de Montevideo, Cell Biology Unit, Montevideo, Uruguay; SERGIO ANCHETA, Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; KAREN PERELMUTER, Institut Pasteur de Montevideo, Cell Biology Unit, Montevideo, Uruguay; ALEJANDRO RICCIARDI; MARIELA BOLLATI-FOGOLÍN, Institut Pasteur de Montevideo, Cell Biology Unit, Montevideo, Uruguay; MARTINA CRISPO, Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay. |
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Título : |
3R's applied to in vivo biological activity of recombinant human erythropoietin assay. |
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Complemento del título : |
Technical Article. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Biological Models Research and Technology Journal. 2023, Volume 3, Issue 1, e00012023. http://dx.doi.org/10.4322/2675-9225.00012023 -- OPEN ACCESS. |
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ISSN : |
2675-9225 |
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DOI : |
10.4322/2675-9225.00012023 |
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Idioma : |
Inglés |
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Notas : |
Article history: Submitted date 9 February 2023, Accepted date 19 April 2023. -- Correspondence: Crispo, M.; Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; email:crispo@pasteur.edu.uy -- FUNDING: This work was supported by FOCEM (MERCOSUR Structural Convergence Fund), COF 03/11 and ANII (EQL_2013_X_1_2). -- Document type: Article Bronze Open Access. --
Publisher: Brazilian Society for Laboratory Animal Science. |
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Contenido : |
ABSTRACT.- An enhanced protocol for the evaluation of in vivo biological activity of recombinant human erythropoietin (rhEPO) assay in mice is described, following European Pharmacopoeia (Ph. Eur.) version 10.0 guideline and optimized by applying the principles of Replacement, Reduction and Refinement (3Rs) in animal experimentation. In Laboratory Animal Science, one of the main principles is to optimize the experimental protocols in order to achieve the best results with the lowest number of animals and refine the procedures to avoid unnecessary suffering. The main objective of this protocol is to comply with international guidelines for rhEPO evaluation, applying refinement on procedures and reduction in animal use. Some of the features included in this protocol are the increase in number of rhEPO batches tested simultaneously against an international standard, leading to a substantial reduction in the number of animal's used, and refinement on animal handling techniques for subcutaneous drug administration and blood withdrawal. The implemented improvements were validated by reticulocyte estimation to ensure compliance with international criteria established for this trial and institutional quality management system. © 2023, Brazilian Society for Laboratory Animal Science. All rights reserved. |
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Palabras claves : |
3R’s; Biological activity; Erythropoietin; European Pharmacopeia; Mice; UNIDAD MIXTA PASTEUR + INIA. |
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Asunto categoría : |
A50 Investigación agraria |
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URL : |
https://bmrtsbcaljournal.com/article/10.4322/2675-9225.00012023/pdf/bmrt-3-1-e00012023.pdf
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Marc : |
LEADER 02721naa a2200301 a 4500
001 1064596
005 2024-04-17
008 2023 bl uuuu u00u1 u #d
022 $a2675-9225
024 7 $a10.4322/2675-9225.00012023$2DOI
100 1 $aAREVALO, A.P.
245 $a3R's applied to in vivo biological activity of recombinant human erythropoietin assay.$h[electronic resource]
260 $c2023
500 $aArticle history: Submitted date 9 February 2023, Accepted date 19 April 2023. -- Correspondence: Crispo, M.; Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; email:crispo@pasteur.edu.uy -- FUNDING: This work was supported by FOCEM (MERCOSUR Structural Convergence Fund), COF 03/11 and ANII (EQL_2013_X_1_2). -- Document type: Article Bronze Open Access. -- Publisher: Brazilian Society for Laboratory Animal Science.
520 $aABSTRACT.- An enhanced protocol for the evaluation of in vivo biological activity of recombinant human erythropoietin (rhEPO) assay in mice is described, following European Pharmacopoeia (Ph. Eur.) version 10.0 guideline and optimized by applying the principles of Replacement, Reduction and Refinement (3Rs) in animal experimentation. In Laboratory Animal Science, one of the main principles is to optimize the experimental protocols in order to achieve the best results with the lowest number of animals and refine the procedures to avoid unnecessary suffering. The main objective of this protocol is to comply with international guidelines for rhEPO evaluation, applying refinement on procedures and reduction in animal use. Some of the features included in this protocol are the increase in number of rhEPO batches tested simultaneously against an international standard, leading to a substantial reduction in the number of animal's used, and refinement on animal handling techniques for subcutaneous drug administration and blood withdrawal. The implemented improvements were validated by reticulocyte estimation to ensure compliance with international criteria established for this trial and institutional quality management system. © 2023, Brazilian Society for Laboratory Animal Science. All rights reserved.
653 $a3R’s
653 $aBiological activity
653 $aErythropoietin
653 $aEuropean Pharmacopeia
653 $aMice
653 $aUNIDAD MIXTA PASTEUR + INIA
700 1 $aBASIKA, T.
700 1 $aANCHETA, S.
700 1 $aPERELMUTER, K.
700 1 $aRICCIARDI, A.
700 1 $aBOLLATI-FOGOLÍN, M.
700 1 $aCRISPO, M.
773 $tBiological Models Research and Technology Journal. 2023, Volume 3, Issue 1, e00012023. http://dx.doi.org/10.4322/2675-9225.00012023 -- OPEN ACCESS.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó; INIA Treinta y Tres. |
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Fecha actual : |
24/08/2016 |
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Actualizado : |
29/05/2018 |
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Tipo de producción científica : |
Documentos |
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Autor : |
BLANCO, P.H.; MARTÍNEZ, S.; CARRACELAS, G.; VARGAS, J.; VILLALBA, M.; ESCALANTE, F. |
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Afiliación : |
PEDRO HORACIO BLANCO BARRAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SEBASTIÁN MARTÍNEZ KOPP, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JULIO GONZALO CARRACELAS GARRIDO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JOSE EDUARDO VARGAS MANCUELLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIO VILLALBA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO DANIEL ESCALANTE UBIEDO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Evaluación de cultivares de calidad americana - E6. |
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Fecha de publicación : |
2016 |
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Fuente / Imprenta : |
ln: JORNADA ANUAL ARROZ, 2016, INIA TREINTA Y TRES, TREINTA Y TRES, UY. Arroz: resultados experimentales 2015-2016. Treinta y Tres, (Uruguay): INIA, 2016. |
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Páginas : |
cap. 3, p. 19-21. |
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Serie : |
(INIA Serie Actividades de Difusión; 765) |
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ISSN : |
1688-9258 |
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Idioma : |
Español |
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Palabras claves : |
JAPÓNICA TROPICAL. |
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Thesagro : |
ARROZ; FITOMEJORAMIENTO. |
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Asunto categoría : |
--
F30 Genética vegetal y fitomejoramiento |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/7881/1/ad-765-cap.3-p.19-21.pdf
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Marc : |
LEADER 00768naa a2200241 a 4500
001 1056562
005 2018-05-29
008 2016 bl uuuu u00u1 u #d
022 $a1688-9258
100 1 $aBLANCO, P.H.
245 $aEvaluación de cultivares de calidad americana - E6.
260 $c2016
300 $acap. 3, p. 19-21.
490 $a(INIA Serie Actividades de Difusión; 765)
650 $aARROZ
650 $aFITOMEJORAMIENTO
653 $aJAPÓNICA TROPICAL
700 1 $aMARTÍNEZ, S.
700 1 $aCARRACELAS, G.
700 1 $aVARGAS, J.
700 1 $aVILLALBA, M.
700 1 $aESCALANTE, F.
773 $tln: JORNADA ANUAL ARROZ, 2016, INIA TREINTA Y TRES, TREINTA Y TRES, UY. Arroz: resultados experimentales 2015-2016. Treinta y Tres, (Uruguay): INIA, 2016.
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INIA Tacuarembó (TBO) |
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